Recombinant human PTDSR/JMJD6 protein

JMJD6 also known as bifunctional arginine demethylase and lysyl-hydroxylase JMJD6. JMJD6 acts as a lysyl-hydroxylase that catalyzes 5-hydroxylation on specific lysine residues of target proteins such as u2AF2/u2AF65 and LuC7L2, as a regulator of RNA splicing by mediating 5-hydroxylation of u2AF2/u2AF65, affecting the pre-mRNA splicing activity of u2AF2/u2AF65. In addition to peptidyl-lysine 5-dioxygenase activity, may act as an RNA hydroxylase, as suggested by its ability to bind single strand RNA. Also acts as an arginine demethylase which demethylates histone H3 at 'Arg-2' (H3R2me) and histone H4 at 'Arg-3' (H4R3me), thereby playing a role in histone code. Recombinant human JMJD6, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Jumonji domain containing 6, Arginine demethylase and lysine hydroxylase, Phosphatidylserine receptor, KIAA0585

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

50kDa (437aa)

Additionnal Information

Jumonji domain containing 6, Arginine demethylase and lysine hydroxylase, Phosphatidylserine receptor, KIAA0585, Bifunctional arginine demethylase and lysyl-hydroxylase JMJD6 isoform1, PSR, PTDSR, PTDSR1, JMJD6, PTDSR 1, JMJD 6, Bifunctional arginine demethylase and lysyl hydroxylase JMJD6 isoform1, ATGP2999-10 µg, ATGP2999-20 µg, ATGP2999-50 µg, ATGP2999-100 µg, ATGP2999-250 µg, ATGP2999-500 µg, ATGP2999-1 mg, ATGP2999-010, ATGP2999-020, ATGP2999-050, ATGP2999-100, ATGP2999-250, ATGP2999-500, ATGP2999-01M

References & Citations

Hong X. et al. (2010) Proc. Natl. Acad. Sci. u.S.A. 107:14568-14572.; ; Webby C.J. et al. (2009) Science 325:90-93.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 30% glycerol, 1mM DTT

Scientific Category

Apoptosis

NCBI Accession Number

NP_001074930

Uniprot Accession Number

Q6NYC1

Species

Human

AA Sequence

MNHKSKK RIREAKRSAR PELKDSLDWT RHNYYESFSL SPAAVADNVE RADALQLSVE EFVERYERPY KPVVLLNAQE GWSAQEKWTL ERLKRKYRNQ KFKCGEDNDG YSVKMKMKYY IEYMESTRDD SPLYIFDSSY GEHPKRRKLL EDYKVPKFFT DDLFQYAGEK RRPPYRWFVM GPPRSGTGIH IDPLGTSAWN ALVQGHKRWC LFPTSTPREL IKVTRDEGGN QQDEAITWFN VIYPRTQLPT WPPEFKPLEI LQKPGETVFV PGGWWHVVLN LDTTIAITQN FASSTNFPVV WHKTVRGRPK LSRKWYRILK QEHPELAVLA DSVDLQESTG IASDSSSDSS SSSSSSSSDS DSECESGSEG DGTVHRRKKR RTCSMVGNGD TTSQDDCVSK ERSSSRIRDT CGGRAHP