Recombinant human CYP2E1 protein

CYP2E1 also known as Cytochrome P450 2E1 (abbreviated CYP2E1, EC 1. 14. 13. n7), is member of the cytochrome P450 mixed-function oxidase system that involved in the metabolism of xenobiotics in the body. While it is involved in the oxidative metabolism of a small range of substrates (mostly small polar molecules), there are many important drug interactions mediated by CYP2E1. Recombinant human CYP2E1, fused to His-tag at N-terminus, was expressed in E. coli.

Product Specifications

Product Name Alternative

Cytochrome P450 2E1, CPE1, CYP2E, P450-J, P450C2E

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Denatured

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 80% by SDS-PAGE

Molecular Weight

56.2 kDa (488aa)

Additionnal Information

CYP2E1, Cytochrome P450 2E1, CPE1, CYP2E, P450-J, P450C2E, ATGP2993-10 µg, ATGP2993-20 µg, ATGP2993-50 µg, ATGP2993-100 µg, ATGP2993-250 µg, ATGP2993-500 µg, ATGP2993-1 mg, ATGP2993-10, ATGP2993-20, ATGP2993-50, ATGP2993-100, ATGP2993-250, ATGP2993-500, ATGP2993-1

References & Citations

Miller DN., et al. (1965) Ann NY Acad Sci, 119 (3) : 957-973.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol

Scientific Category

Cardiovascular

NCBI Accession Number

NP_000764.1

Uniprot Accession Number

P05181

Species

Human

AA Sequence

SWNLPPG PFPLPIIGNL FQLELKNIPK SFTRLAQRFG PVFTLYVGSQ RMVVMHGYKA VKEALLDYKD EFSGRGDLPA FHAHRDRGII FNNGPTWKDI RRFSLTTLRN YGMGKQGNES RIQREAHFLL EALRKTQGQP FDPTFLIGCA PCNVIADILF RKHFDYNDEK FLRLMYLFNE NFHLLSTPWL QLYNNFPSFL HYLPGSHRKV IKNVAEVKEY VSERVKEHHQ SLDPNCPRDL TDCLLVEMEK EKHSAERLYT MDGITVTVAD LFFAGTETTS TTLRYGLLIL MKYPEIEEKL HEEIDRVIGP SRIPAIKDRQ EMPYMDAVVH EIQRFITLVP SNLPHEATRD TIFRGYLIPK GTVVVPTLDS VLYDNQEFPD PEKFKPEHFL NENGKFKYSD YFKPFSTGKR VCAGEGLARM ELFLLLCAIL QHFNLKPLVD PKDIDLSPIH IGFGCIPPRY KLCVIPRS