Recombinant human IGFBP-3 protein

IGFBP3 also known as Insulin-like growth factor binding protein 3 isoform b precursor. They are structurally and functionally related to insulin but have a much higher growth-promoting activity. It may be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. It stimulates glucose transport in rat bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake. It may play a role in synapse maturation. Recombinant human IGFBP3, fused to His-tag at N-terminus, was expressed in E. coli.

Product Specifications

Product Name Alternative

Insulin-like growth factor binding protein 3 isoform b precursor, IBP3, BP-53

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Denatured

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

31 kDa (285aa)

Additionnal Information

IGFBP3, Insulin-like growth factor binding protein 3 isoform b precursor, IBP3, BP-53, ATGP2991-10 µg, ATGP2991-20 µg, ATGP2991-50 µg, ATGP2991-100 µg, ATGP2991-250 µg, ATGP2991-500 µg, ATGP2991-1 mg, ATGP2991-10, ATGP2991-20, ATGP2991-50, ATGP2991-100, ATGP2991-250, ATGP2991-500, ATGP2991-1

References & Citations

Zoidis E., et al. (2011) Mol. Cell. Biochem. 348:33-42

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol

Scientific Category

Apoptosis

NCBI Accession Number

NP_000589

Uniprot Accession Number

P17936

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGASSAGLGP VVRCEPCDAR ALAQCAPPPA VCAELVREPG CGCCLTCALS EGQPCGIYTE RCGSGLRCQP SPDEARPLQA LLDGRGLCVN ASAVSRLRAY LLPAPPAPGN ASESEEDRSA GSVESPSVSS THRVSDPKFH PLHSKIIIIK KGHAKDSQRY KVDYESQSTD TQNFSSESKR ETEYGPCRRE MEDTLNHLKF LNVLSPRGVH IPNCDKKGFY KKKQCRPSKG RKRGFCWCVD KYGQPLPGYT TKGKEDVHCY SMQSK