Recombinant mouse IL-2 (C160S) protein

IL2 (Interleukin2), also known as T cell growth factor (TCGF), is an immunoregulatory lymphokine produced by T-cells in response to antigenic or mitogenic stimulation. IL2/IL-2R signaling is required for T-cell proliferation and other fundamental functions which are essential for the immune response. IL2 stimulates growth and differentiation of B-cells, NK cells, lymphokine activated killer cells, monocytes, macrophages and oligodendrocytes. Recombinant mouse IL2 was expressed in E. coli and purified by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.

Product Specifications

Specifications

Mouse IL-2 (C160S) stimulates cell proliferation of the CTLL2 mouse cytotoxic T cells. The ED50 range ≤0.9 ng/mL.

Product Name Alternative

Interleukin-2, IL-2, T-cell growth factor, TCGF

Expression System

E.coli

Antigen Species

Mouse

Tag

Non-Tagged

Applications

SDS-PAGE, Bioactivity

Concentration

0.5 mg/mL (determined by BCA assay)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 90% by SDS-PAGE

Bioactivity

Measured in a cell proliferation assay using CTLL-2 mouse cytotoxic T cells. The ED50 range ≤0.9 ng/mL.

Molecular Weight

17.3 kDa (150aa) confirmed by MALDI-TOF

Additionnal Information

ILB0905, C160S, IL 2, IL2, IL-2, Interleukin 2, Interleukin2, Interleukin-2, T cell growth factor, Tcell growth factor, T-cell growth factor, TCGF, ATGP2987-10 µg, ATGP2987-20 µg, ATGP2987-50 µg, ATGP2987-100 µg, ATGP2987-250 µg, ATGP2987-500 µg, ATGP2987-1 mg, ATGP2987-010, ATGP2987-020, ATGP2987-050, ATGP2987-100, ATGP2987-250, ATGP2987-500, ATGP2987-01M

References & Citations

Watson J., et al. (1980) . Immunol Rev. 51:257-278.; ; Taniguchi T., et al. (1983) . Nature. 302: 305-310.

Other References

Hyung KE, et al. Lactobacillus plantarum isolated from kimchi regulates inflammation by increasing interleukin-10 secretion by antigen-presenting cells, leading to diminishing of STAT5 phosphorylation in Th2 cells. (J Food Sci. 2024) {https://pubmed.ncbi.nlm.nih.gov/38685880/}

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) 10% glycerol

Scientific Category

Hormones & Cytokines

NCBI Accession Number

NP_032392

Uniprot Accession Number

P04351

Species

Mouse

AA Sequence

MAPTSSSTSS STAEAQQQQQ QQQQQQQHLE QLLMDLQELL SRMENYRNLK LPRMLTFKFY LPKQATELKD LQCLEDELGP LRHVLDLTQS KSFQLEDAEN FISNIRVTVV KLKGSDNTFE CQFDDESATV VDFLRRWIAF SQSIISTSPQ