Recombinant human IMPAD1 protein

IMPAD1, also known as Inositol monophosphatase 3, is a member of the inositol monophosphatase family. IMPAD1 is localized to the Golgi apparatus and catalyzes the hydrolysis of phosphoadenosine phosphate (PAP) to adenosine monophosphate (AMP) . Mutations in this gene are a cause of GRAPP type chondrodysplasia with joint dislocations, and a pseudogene of this gene is located on the long arm of chromosome 1. Recombinant human IMPAD1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.

Product Specifications

Specifications

Specific acitivty > 3300 pmol/min/ug, its ability to dephosphorylate adenosine 3'5'-diphosphate sodium slat at pH 7.5, 25C.

Product Name Alternative

Inositol monophosphatase 3, GPAPP, IMP 3, IMP-3, IMPA3

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

Enzyme Activity, SDS-PAGE

Concentration

0.25 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Bioactivity

Specific acitivty > 3300pmol/min/ug, its ability to dephosphorylate adenosine 3'5'-diphosphate sodium slat at pH 7.5, 25C.

Molecular Weight

37.6 kDa (349aa) confirmed by MALDI-TOF

Additionnal Information

IMPAD1, Inositol monophosphatase 3, GPAPP, IMP 3, IMP-3, IMPA3, ATGP2986-10 µg, ATGP2986-20 µg, ATGP2986-50 µg, ATGP2986-100 µg, ATGP2986-250 µg, ATGP2986-500 µg, ATGP2986-1 mg, ATGP2986-10, ATGP2986-20, ATGP2986-50, ATGP2986-100, ATGP2986-250, ATGP2986-500, ATGP2986-1

References & Citations

Vissers L E., et al. (2011) Am J Hum Genet. 88:608-615. ; ; Kalujnaia S., et al. (2010) FASEB J. 24:3981-3991.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4)

Scientific Category

Cardiovascular

NCBI Accession Number

NP_060283

Uniprot Accession Number

Q9NX62

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGSGRFSLFG LGGEPGGGAA GPAAAADGGT VDLREMLAVS VLAAVRGGDE VRRVRESNVL HEKSKGKTRE GAEDKMTSGD VLSNRKMFYL LKTAFPSVQI NTEEHVDAAD QEVILWDHKI PEDILKEVTT PKEVPAESVT VWIDPLDATQ EYTEDLRKYV TTMVCVAVNG KPMLGVIHKP FSEYTAWAMV DGGSNVKARS SYNEKTPRIV VSRSHSGMVK QVALQTFGNQ TTIIPAGGAG YKVLALLDVP DKSQEKADLY IHVTYIKKWD ICAGNAILKA LGGHMTTLSG EEISYTGSDG IEGGLLASIR MNHQALVRKL PDLEKTGHK

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