Recombinant E.coli Cyclophilin A/PPIA protein

PpiA also kmown as Peptidyl-prolyl cis-trans isomerase A. This protein is PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. Recombinant E. coli ppiA protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Rotamase A, rotA, rot, PPIase A, Peptidyl-prolyl cis-trans isomerase A, Cyclophilin A

Expression System

E.coli

Antigen Species

E.coli

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Absorbance at 280 nm)

Purity

> 95% by SDS-PAGE

Molecular Weight

20.5 kDa (189aa) confirmed by MALDI-TOF

Additionnal Information

Cyclophilin A, ppiA, ATGP2983-10 µg, ATGP2983-20 µg, ATGP2983-50 µg, ATGP2983-100 µg, ATGP2983-250 µg, ATGP2983-500 µg, ATGP2983-1 mg, ATGP2983-10, ATGP2983-20, ATGP2983-50, ATGP2983-100, ATGP2983-250, ATGP2983-500, ATGP2983-1

References & Citations

Liu J., et al. (1990) Proc. Natl. Acad. Sci. u.S.A. 87:4028-4032.; ; Hayano T., et al. (1991) Biochemistry 30:3041-3048.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Scientific Category

Cell Cycle

NCBI Accession Number

NP_417822.1

Uniprot Accession Number

P0AFL3

Species

E.coli

AA Sequence

AKGDPHV LLTTSAGNIE LELDKQKAPV SVQNFVDYVN SGFYNNTTFH RVIPGFMIQG GGFTEQMQQK KPNPPIKNEA DNGLRNTRGT IAMARTADKD SATSQFFINV ADNAFLDHGQ RDFGYAVFGK VVKGMDVADK ISQVPTHDVG PYQNVPSKPV VILSAKVLP