Recombinant E.coli Cyclophilin A/PPIA protein
PpiA also kmown as Peptidyl-prolyl cis-trans isomerase A. This protein is PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. Recombinant E. coli ppiA protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Rotamase A, rotA, rot, PPIase A, Peptidyl-prolyl cis-trans isomerase A, Cyclophilin A
Expression System
E.coli
Antigen Species
E.coli
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Absorbance at 280 nm)
Purity
> 95% by SDS-PAGE
Molecular Weight
20.5 kDa (189aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Liu J., et al. (1990) Proc. Natl. Acad. Sci. u.S.A. 87:4028-4032.; ; Hayano T., et al. (1991) Biochemistry 30:3041-3048.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol
Scientific Category
Cell Cycle
NCBI Accession Number
NP_417822.1
Uniprot Accession Number
P0AFL3
Species
E.coli
AA Sequence
Frequently Asked Questions
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