Recombinant mouse Aldo-keto reductase 1B1/AKR1B1 protein
Akr1b1, also known as Aldose reductas, is an enzyme. It catalyzes the NADPH-dependent reduction of a wide variety of carbonyl-containing compounds to their corresponding alcohols with a broad range of catalytic efficiencies. Recombinant mouse Akr1b1, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Specifications
Product Name Alternative
Expression System
E.coli
Antigen Species
Mouse
Tag
His-Tag
Applications
SDS-PAGE, Enzyme Activity
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 95% by SDS-PAGE
Bioactivity
Molecular Weight
38.1 kDa (339aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Wilson DK, Bohren KM, et al. (1992) . Science. 257 (5066) :81-4.; ; Graham A, Brown L, et al. (1992) . J Biol Chem. 266 (11) :6872-7.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol
Scientific Category
Metabolism
NCBI Accession Number
NP_033788
Uniprot Accession Number
P45376
Species
Mouse
AA Sequence
Frequently Asked Questions
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