Recombinant mouse Aldo-keto reductase 1B1/AKR1B1 protein

Akr1b1, also known as Aldose reductas, is an enzyme. It catalyzes the NADPH-dependent reduction of a wide variety of carbonyl-containing compounds to their corresponding alcohols with a broad range of catalytic efficiencies. Recombinant mouse Akr1b1, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Specifications

Specific activity is > 500 pmol/min/ug, and is defined as the amount of enzyme that catalyze the reduction of 1.0 pmole DL-glyceraldehyde in the presence of NADPH per minute at pH7.0 at 37C.

Product Name Alternative

Aldose reductase, Ahr-1, Ahr1, Akr1b1, Aldor1, Aldr1, ALR2, AR, Aldo-keto reductase family 1 member B1, Aldehyde reductase, Aldo-keto reductase family 1 member B3, Akr1b3

Expression System

E.coli

Antigen Species

Mouse

Tag

His-Tag

Applications

SDS-PAGE, Enzyme Activity

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Bioactivity

Specific activity is > 500pmol/min/ug, and is defined as the amount of enzyme that catalyze the reduction of 1.0pmole DL-glyceraldehyde in the presence of NADPH per minute at pH7.0 at 37C.

Molecular Weight

38.1 kDa (339aa) confirmed by MALDI-TOF

Additionnal Information

Aldose reductase, Akr1b1, Aldose reductase, Ahr-1, Ahr1, Akr1b1, Aldor1, Aldr1, ALR2, AR, ATGP2982-10 µg, ATGP2982-20 µg, ATGP2982-50 µg, ATGP2982-100 µg, ATGP2982-250 µg, ATGP2982-500 µg, ATGP2982-1 mg, ATGP2982-10, ATGP2982-20, ATGP2982-50, ATGP2982-100, ATGP2982-250, ATGP2982-500, ATGP2982-1

References & Citations

Wilson DK, Bohren KM, et al. (1992) . Science. 257 (5066) :81-4.; ; Graham A, Brown L, et al. (1992) . J Biol Chem. 266 (11) :6872-7.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Scientific Category

Metabolism

NCBI Accession Number

NP_033788

Uniprot Accession Number

P45376

Species

Mouse

AA Sequence

MASHLEL NNGTKMPTLG LGTWKSPPGQ VTEAVKVAID LGYRHIDCAQ VYQNEKEVGV ALQEKLKEQV VKRQDLFIVS KLWCTFHDKS MVKGAFQKTL SDLQLDYLDL YLIHWPTGFK PGPDYFPLDA SGNVIPSDTD FVDTWTAMEQ LVDEGLVKTI GVSNFNPLQI ERILNKPGLK YKPAVNQIEC HPYLTQEKLI EYCHSKGIVV TAYSPLGSPD RPWAKPEDPS LLEDPRIKAI AAKYNKTTAQ VLIRFPIQRN LVVIPKSVTP VRIAENLKVF DFEVSSEDMA TLLSYNRNWR VCALMSCAKH KDYPFHAEV