Recombinant human PEPD protein
PEPD also known as Xaa-Pro dipeptidase isoform1. The protein forms a homodimer that hydrolyzes dipeptides or tripeptides with C-terminal proline or hydroxyproline residues. The enzyme serves an important role in the recycling of proline, and may be rate limiting for the production of collagen. Mutations in this gene result in prolidase deficiency, which is characterized by the excretion of large amount of di- and tri-peptides containing proline. Recombinant human PEPD, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Xaa-Pro dipeptidase isoform 1, PROLIDASE
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
56.9 kDa (516aa)
Additionnal Information
References & Citations
Toprak G., et al. (2013) Eur Rev Med Pharmacol Sci 17 (17), 2302-2309; ; Surazynski A., et al. (2013) Mol. Cell. Biochem. 379 (1-2), 29-36
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol, 1mM DTT
Scientific Category
Metabolism
NCBI Accession Number
NP_000276
Uniprot Accession Number
P12955
Species
Human
AA Sequence
Frequently Asked Questions
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