Recombinant human PEPD protein

PEPD also known as Xaa-Pro dipeptidase isoform1. The protein forms a homodimer that hydrolyzes dipeptides or tripeptides with C-terminal proline or hydroxyproline residues. The enzyme serves an important role in the recycling of proline, and may be rate limiting for the production of collagen. Mutations in this gene result in prolidase deficiency, which is characterized by the excretion of large amount of di- and tri-peptides containing proline. Recombinant human PEPD, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Xaa-Pro dipeptidase isoform 1, PROLIDASE

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

56.9 kDa (516aa)

Additionnal Information

PEPD, Xaa-Pro dipeptidase isoform 1, PROLIDASE, ATGP2980-10 µg, ATGP2980-20 µg, ATGP2980-50 µg, ATGP2980-100 µg, ATGP2980-250 µg, ATGP2980-500 µg, ATGP2980-1 mg, ATGP2980-10, ATGP2980-20, ATGP2980-50, ATGP2980-100, ATGP2980-250, ATGP2980-500, ATGP2980-1

References & Citations

Toprak G., et al. (2013) Eur Rev Med Pharmacol Sci 17 (17), 2302-2309; ; Surazynski A., et al. (2013) Mol. Cell. Biochem. 379 (1-2), 29-36

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol, 1mM DTT

Scientific Category

Metabolism

NCBI Accession Number

NP_000276

Uniprot Accession Number

P12955

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGSMAAATGP SFWLGNETLK VPLALFALNR QRLCERLRKN PAVQAGSIVV LQGGEETQRY CTDTGVLFRQ ESFFHWAFGV TEPGCYGVID VDTGKSTLFV PRLPASHATW MGKIHSKEHF KEKYAVDDVQ YVDEIASVLT SQKPSVLLTL RGVNTDSGSV CREASFDGIS KFEVNNTILH PEIVECRVFK TDMELEVLRY TNKISSEAHR EVMKAVKVGM KEYELESLFE HYCYSRGGMR HSSYTCICGS GENSAVLHYG HAGAPNDRTI QNGDMCLFDM GGEYYCFASD ITCSFPANGK FTADQKAVYE AVLRSSRAVM GAMKPGVWWP DMHRLADRIH LEELAHMGIL SGSVDAMVQA HLGAVFMPHG LGHFLGIDVH DVGGYPEGVE RIDEPGLRSL RTARHLQPGM VLTVEPGIYF IDHLLDEALA DPARASFLNR EVLQRFRGFG GVRIEEDVVV TDSGIELLTC VPRTVEEIEA CMAGCDKAFT PFSGPK