Recombinant human Granzyme B protein

GZMB also known as granzyme B is crucial for the rapid induction of target cell apoptosis by CTL in cell-mediated immune response. Cytolytic T lymphocytes (CTL) and natural killer (NK) cells share the remarkable ability to recognize, bind, and lyse specific target cells. They are thought to protect their host by lysing cells bearing on their surface nonself antigens, usually peptides or proteins resulting from infection by intracellular pathogens. Recombinant human GZMB, fused to His-tag at N-terminus, was expressed in E. coli.

Product Specifications

Product Name Alternative

Granzyme B, CCPI, CGL-1, CGL1, CSP-B, CSPB, CTLA1, CTSGL1, HLP, SECT

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Denatured

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

27.8 kDa (248aa)

Additionnal Information

Granzyme B, Granzyme B, CCPI, CGL-1, CGL1, CSP-B, CSPB, CTLA1, CTSGL1, HLP, SECT, ATGP2978-10 µg, ATGP2978-20 µg, ATGP2978-50 µg, ATGP2978-100 µg, ATGP2978-250 µg, ATGP2978-500 µg, ATGP2978-1 mg, ATGP2978-10, ATGP2978-20, ATGP2978-50, ATGP2978-100, ATGP2978-250, ATGP2978-500, ATGP2978-1

References & Citations

Xu W., et al. (2014) Eur. J. Immunol. 44 (1), 275-284; ; Fritsch K., et al. (2013) Ann. Hematol. 92 (12), 1603-1609

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol

Scientific Category

Apoptosis

NCBI Accession Number

NP_004122

Uniprot Accession Number

P10144

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MIIGGHEAKP HSRPYMAYLM IWDQKSLKRC GGFLIQDDFV LTAAHCWGSS INVTLGAHNI KEQEPTQQFI PVKRPIPHPA YNPKNFSNDI MLLQLERKAK RTRAVQPLRL PSNKAQVKPG QTCSVAGWGQ TAPLGKHSHT LQEVKMTVQE DRKCESDLRH YYDSTIELCV GDPEIKKTSF KGDSGGPLVC NKVAQGIVSY GRNNGMPPRA CTKVSSFVHW IKKTMKRY