Recombinant human AGA protein

AGA also known as N (4) - (beta-N-acetylglucosaminyl) -L-asparaginase is involved in the catabolism of N-linked oligosaccharides of glycoproteins. It cleaves asparagine from N-acetylglucosamines as one of the final steps in the lysosomal breakdown of glycoproteins. Recombinant human AGA, fused to His-tag at N-terminus, was expressed in E. coli

Product Specifications

Product Name Alternative

N (4) - (beta-N-acetylglucosaminyl) -L-asparaginase isoform 1, AGu, ASRG, GA

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Denatured

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

37 kDa (346aa)

Additionnal Information

AGA, N (4) - (beta-N-acetylglucosaminyl) -L-asparaginase isoform 1, AGu, ASRG, GA, ATGP2977-10 µg, ATGP2977-20 µg, ATGP2977-50 µg, ATGP2977-100 µg, ATGP2977-250 µg, ATGP2977-500 µg, ATGP2977-1 mg, ATGP2977-10, ATGP2977-20, ATGP2977-50, ATGP2977-100, ATGP2977-250, ATGP2977-500, ATGP2977-1

References & Citations

Covini D., et al. (2012) Recent Pat Anticancer Drug Discov 7 (1), 4-13

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol

Scientific Category

Enzymes & Proteases

NCBI Accession Number

NP_000018

Uniprot Accession Number

P20933

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGSSSPLPLV VNTWPFKNAT EAAWRALASG GSALDAVESG CAMCEREQCD GSVGFGGSPD ELGETTLDAM IMDGTTMDVG AVGDLRRIKN AIGVARKVLE HTTHTLLVGE SATTFAQSMG FINEDLSTTA SQALHSDWLA RNCQPNYWRN VIPDPSKYCG PYKPPGILKQ DIPIHKETED DRGHDTIGMV VIHKTGHIAA GTSTNGIKFK IHGRVGDSPI PGAGAYADDT AGAAAATGNG DILMRFLPSY QAVEYMRRGE DPTIACQKVI SRIQKHFPEF FGAVICANVT GSYGAACNKL STFTQFSFMV YNSEKNQPTE EKVDCI