Recombinant human MTHFD2 protein

MTHFD2 also known as Bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase, mitochondrial. MTHFD2 protein that possesses three distinct enzymatic activities, methylenetetrahydrofolate dehydrogenase, methenyltetrahydrofolate cyclohydrolase and formate-tetrahydrofolate ligase. Each of these activities catalyzes one of three sequential reactions in the interconversion of 1-carbon derivatives of tetrahydrofolate, which are substrates for methionine, thymidylate, and de novo purine syntheses. Recombinant human MTHFD2, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase mitochondrial, Bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase, mitochondrial, NMDMC

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

37.2 kDa (344aa) confirmed by MALDI-TOF

Additionnal Information

MTHFD2, Bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase mitochondrial, Bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase, mitochondrial, NMDMC, ATGP2972-10 µg, ATGP2972-20 µg, ATGP2972-50 µg, ATGP2972-100 µg, ATGP2972-250 µg, ATGP2972-500 µg, ATGP2972-1 mg, ATGP2972-10, ATGP2972-20, ATGP2972-50, ATGP2972-100, ATGP2972-250, ATGP2972-500, ATGP2972-1

References & Citations

Watkins D., et al. (2011) Genet. 48 (9) : 590-2.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 20% glycerol, 1mM DTT

Scientific Category

Metabolism

NCBI Accession Number

NP_006627.2

Uniprot Accession Number

P13995

Species

Human

AA Sequence

LAAVRNE AVVISGRKLA QQIKQEVRQE VEEWVASGNK RPHLSVILVG ENPASHSYVL NKTRAAAVVG INSETIMKPA SISEEELLNL INKLNNDDNV DGLLVQLPLP EHIDERRICN AVSPDKDVDG FHVINVGRMC LDQYSMLPAT PWGVWEIIKR TGIPTLGKNV VVAGRSKNVG MPIAMLLHTD GAHERPGGDA TVTISHRYTP KEQLKKHTIL ADIVISAAGI PNLITADMIK EGAAVIDVGI NRVHDPVTAK PKLVGDVDFE GVRQKAGYIT PVPGGVGPMT VAMLMKNTII AAKKVLRLEE REVLKSKELG VATN