Recombinant human PU.1/SPI1 protein
SPI1 also known as transcription factor Pu. 1, is a protein that in humans is encoded by the SPI1 gene. This protein have ETS-domain transcription factor that activates gene expression during myeloid and B-lymphoid cell development. The nuclear protein binds to a purine-rich sequence known as the Pu-box found near the promoters of target genes, and regulates their expression in coordination with other transcription factors and cofactors. It can also regulate alternative splicing of target genes. Multiple transcript variants encoding different isoforms have been found for this gene. Recombinant SPI1, fused to His-tag at N-terminus, was expressed in E. coli.
Product Specifications
Product Name Alternative
Transcription factor Pu.1 isoform 1, OF, Pu.1, SFPI1, SPI-1, SPI-A, Spi-1 proto-oncogene
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE, Denatured
Concentration
0.25 mg/mL (determined by Bradford assay)
Purity
> 85% by SDS-PAGE
Molecular Weight
33.6 kDa (294aa)
Additionnal Information
References & Citations
Ray D, Culine S, et al. (1990) . Oncogene. 5 (5) :663-8.; ; Hallier M, Tavitian A, et al. (1996) . J Biol Chem. 271 (19) :11177-81.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol
Scientific Category
Immunology
NCBI Accession Number
NP_001074016
Uniprot Accession Number
P17947
Species
Human
AA Sequence
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