Recombinant human PU.1/SPI1 protein

SPI1 also known as transcription factor Pu. 1, is a protein that in humans is encoded by the SPI1 gene. This protein have ETS-domain transcription factor that activates gene expression during myeloid and B-lymphoid cell development. The nuclear protein binds to a purine-rich sequence known as the Pu-box found near the promoters of target genes, and regulates their expression in coordination with other transcription factors and cofactors. It can also regulate alternative splicing of target genes. Multiple transcript variants encoding different isoforms have been found for this gene. Recombinant SPI1, fused to His-tag at N-terminus, was expressed in E. coli.

Product Specifications

Product Name Alternative

Transcription factor Pu.1 isoform 1, OF, Pu.1, SFPI1, SPI-1, SPI-A, Spi-1 proto-oncogene

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Denatured

Concentration

0.25 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

33.6 kDa (294aa)

Additionnal Information

SPI1, Transcription factor Pu.1 isoform 1, OF, Pu.1, SFPI1, SPI-1, SPI-A, ATGP2969-10 µg, ATGP2969-20 µg, ATGP2969-50 µg, ATGP2969-100 µg, ATGP2969-250 µg, ATGP2969-500 µg, ATGP2969-1 mg, ATGP2969-10, ATGP2969-20, ATGP2969-50, ATGP2969-100, ATGP2969-250, ATGP2969-500, ATGP2969-1

References & Citations

Ray D, Culine S, et al. (1990) . Oncogene. 5 (5) :663-8.; ; Hallier M, Tavitian A, et al. (1996) . J Biol Chem. 271 (19) :11177-81.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol

Scientific Category

Immunology

NCBI Accession Number

NP_001074016

Uniprot Accession Number

P17947

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGSMLQACKM EGFPLVPPQP SEDLVPYDTD LYQRQTHEYY PYLSSDGESH SDHYWDFHPH HVHSEFESFA ENNFTELQSV QPPQLQQLYR HMELEQMHVL DTPMVPPHPS LGHQVSYLPR MCLQYPSLSP AQPSSDEEEG ERQSPPLEVS DGEADGLEPG PGLLPGETGS KKKIRLYQFL LDLLRSGDMK DSIWWVDKDK GTFQFSSKHK EALAHRWGIQ KGNRKKMTYQ KMARALRNYG KTGEVKKVKK KLTYQFSGEV LGRGGLAERR HPPH