Recombinant human Follistatin/FST protein

FST, also known as Follistatin, is a single-chain gonadal protein that specifically inhibits follicle-stimulating hormone release. The single FST gene encodes two isoforms, FST317 and FST344 containing 317 and 344 amino acids respectively, resulting from alternative splicing of the precursor mRNA. Recombinant human FST, fused to His-tag at C-terminus, was expressed in Sf9 and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Follistatin isoform FST317, FS

Expression System

Baculovirus

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by Bradford assay)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 90% by SDS-PAGE

Molecular Weight

32.5 kDa (295aa) 40-57 kDa, reducing conditions

Additionnal Information

Follistatin (aa300344), Follistatin isoform FST344, FS, ATGP2964-10 µg, ATGP2964-20 µg, ATGP2964-50 µg, ATGP2964-100 µg, ATGP2964-250 µg, ATGP2964-500 µg, ATGP2964-1 mg, ATGP2964-10, ATGP2964-20, ATGP2964-50, ATGP2964-100, ATGP2964-250, ATGP2964-500, ATGP2964-1

References & Citations

Sang Q, Zhang S, et al. (2013) . Reprod Biomed Online. 26 (2) :157-63.; ; Akiyama I, Yoshino O, et al. (2013) . Reprod Sci. 20 (6) :675-9.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Scientific Category

Signal Transduction

NCBI Accession Number

NP_006341

Uniprot Accession Number

P19883

Species

Human

AA Sequence

MGNCWLRQAK NGRCQVLYKT ELSKEECCST GRLSTSWTEE DVNDNTLFKW MIFNGGAPNC IPCKETCENV DCGPGKKCRM NKKNKPRCVC APDCSNITWK GPVCGLDGKT YRNECALLKA RCKEQPELEV QYQGRCKKTC RDVFCPGSST CVVDQTNNAY CVTCNRICPE PASSEQYLCG NDGVTYSSAC HLRKATCLLG RSIGLAYEGK CIKAKSCEDI QCTGGKKCLW DFKVGRGRCS LCDELCPDSK SDEPVCASDN ATYASECAMK EAACSSGVLL EVKHSGSCNH HHHHH