Recombinant E.coli tnaA protein

TnaA, also known as Tryptophanase/L-cysteine desulfhydrase. Tryptophanase is an enzyme that catalyzes the chemical reaction. Thus, the two substrates of this enzyme are L-tryptophan and H2O, whereas its 3 products are indole, pyruvate, and NH3. Recombinant E. coli tnaA, fused to His-tag at N-terminus, was expressed in E. coli and purified using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Tryptophanase/L-cysteine desulfhydrase PLP-dependent, Tryptophanase/L-cysteine desulfhydrase, PLP-dependent, Tryptophanase

Expression System

E.coli

Antigen Species

E.coli

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

55.2 kDa (494aa)

Additionnal Information

TnaA, ATGP2963-10 µg, ATGP2963-20 µg, ATGP2963-50 µg, ATGP2963-100 µg, ATGP2963-250 µg, ATGP2963-500 µg, ATGP2963-1 mg, ATGP2963-10, ATGP2963-20, ATGP2963-50, ATGP2963-100, ATGP2963-250, ATGP2963-500, ATGP2963-1

References & Citations

Tokushige M., et al. (1989) Biochimie 71:711-720

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol, 1mM DTT

Scientific Category

Enzymes & Proteases

NCBI Accession Number

NP_418164

Uniprot Accession Number

P0A853

Species

E.coli

AA Sequence

MENFKHL PEPFRIRVIE PVKRTTRAYR EEAIIKSGMN PFLLDSEDVF IDLLTDSGTG AVTQSMQAAM MRGDEAYSGS RSYYALAESV KNIFGYQYTI PTHQGRGAEQ IYIPVLIKKR EQEKGLDRSK MVAFSNYFFD TTQGHSQING CTVRNVYIKE AFDTGVRYDF KGNFDLEGLE RGIEEVGPNN VPYIVATITS NSAGGQPVSL ANLKAMYSIA KKYDIPVVMD SARFAENAYF IKQREAEYKD WTIEQITRET YKYADMLAMS AKKDAMVPMG GLLCMKDDSF FDVYTECRTL CVVQEGFPTY GGLEGGAMER LAVGLYDGMN LDWLAYRIAQ VQYLVDGLEE IGVVCQQAGG HAAFVDAGKL LPHIPADQFP AQALACELYK VAGIRAVEIG SFLLGRDPKT GKQLPCPAEL LRLTIPRATY TQTHMDFIIE AFKHVKENAA NIKGLTFTYE PKVLRHFTAK LKEV