Recombinant human PRMT3 protein

PRMT3, as known as protein arginine N-methyltransferase 3, belongs to the protein arginine methyltransferase family. This enzyme catalyzes the methylation of guanidino nitrogens of arginyl residues of proteins. It acts on 40S ribosomal protein S2 (rpS2), which is its major in-vivo substrate, and is involved in the proper maturation of the 80S ribosome. Recombinant human PRMT3, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Protein arginine N-methyltransferase 3 isoform 1, HRMT1L3

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

62.3 kDa (554aa)

Additionnal Information

PRMT3, Protein arginine N-methyltransferase 3 isoform 1, HRMT1L3, ATGP2958-10 µg, ATGP2958-20 µg, ATGP2958-50 µg, ATGP2958-100 µg, ATGP2958-250 µg, ATGP2958-500 µg, ATGP2958-1 mg, ATGP2958-10, ATGP2958-20, ATGP2958-50, ATGP2958-100, ATGP2958-250, ATGP2958-500, ATGP2958-1

References & Citations

Tang J, Gary JD, et al. (1998) . J Biol Chem. 273 (27) :16935-45.; ; Choi S, Jung CR, et al. (2008) . Biochim Biophys Acta. 1780 (9) :1062-9.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 20% glycerol

Scientific Category

Signal Transduction

NCBI Accession Number

NP_005779.1

Uniprot Accession Number

O60678

Species

Human

AA Sequence

MCSLASG ATGGRGAVEN EEDLPELSDS GDEAAWEDED DADLPHGKQQ TPCLFCNRLF TSAEETFSHC KSEHQFNIDS MVHKHGLEFY GYIKLINFIR LKNPTVEYMN SIYNPVPWEK EEYLKPVLED DLLLQFDVED LYEPVSVPFS YPNGLSENTS VVEKLKHMEA RALSAEAALA RAREDLQKMK QFAQDFVMHT DVRTCSSSTS VIADLQEDED GVYFSSYGHY GIHEEMLKDK IRTESYRDFI YQNPHIFKDK VVLDVGCGTG ILSMFAAKAG AKKVLGVDQS EILYQAMDII RLNKLEDTIT LIKGKIEEVH LPVEKVDVII SEWMGYFLLF ESMLDSVLYA KNKYLAKGGS VYPDICTISL VAVSDVNKHA DRIAFWDDVY GFKMSCMKKA VIPEAVVEVL DPKTLISEPC GIKHIDCHTT SISDLEFSSD FTLKITRTSM CTAIAGYFDI YFEKNCHNRV VFSTGPQSTK THWKQTVFLL EKPFSVKAGE ALKGKVTVHK NKKDPRSLTV TLTLNNSTQT YGLQ