Recombinant human PRMT3 protein
PRMT3, as known as protein arginine N-methyltransferase 3, belongs to the protein arginine methyltransferase family. This enzyme catalyzes the methylation of guanidino nitrogens of arginyl residues of proteins. It acts on 40S ribosomal protein S2 (rpS2), which is its major in-vivo substrate, and is involved in the proper maturation of the 80S ribosome. Recombinant human PRMT3, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Protein arginine N-methyltransferase 3 isoform 1, HRMT1L3
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
62.3 kDa (554aa)
Additionnal Information
References & Citations
Tang J, Gary JD, et al. (1998) . J Biol Chem. 273 (27) :16935-45.; ; Choi S, Jung CR, et al. (2008) . Biochim Biophys Acta. 1780 (9) :1062-9.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 20% glycerol
Scientific Category
Signal Transduction
NCBI Accession Number
NP_005779.1
Uniprot Accession Number
O60678
Species
Human
AA Sequence
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