Recombinant human NRF2/NFE2L2 protein

Nuclear factor (erythroid-derived 2) -like 2, also known as NRF2, is bZIP transcription factors that heterodimerize with Maf proteins to bind Mare sequences. The NRF proteins also bind the antioxidant response element (ARE) and are implicated in the regulation of detoxification enzymes and the oxidative stress response. NRF2 is widely expressed and is thought to translocate to the nucleus after treatment with xenobiotics and antioxidants, which stimulate its release from its repressor protein, Keap1. Recombinant human NRF2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

NFE2 like bZIP transcription factor 2, Nuclear factor erythroid 2-related factor 2, NRF-2, NF-E2-related factor 2, HEBP1

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

69.9 kDa (625aa)

Additionnal Information

NRF2, ATGP2948-10 µg, ATGP2948-20 µg, ATGP2948-50 µg, ATGP2948-100 µg, ATGP2948-250 µg, ATGP2948-500 µg, ATGP2948-1 mg, ATGP2948-10, ATGP2948-20, ATGP2948-50, ATGP2948-100, ATGP2948-250, ATGP2948-500, ATGP2948-1

References & Citations

Huang H C., et al. (2000) Proc Natl Acad Sci uSA. 97: 12475-12480.; ; Tsai P Y., et al. (2012) Arthritis Rheum. 64: 232-242.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Scientific Category

Epigenetics and Nuclear Signaling

NCBI Accession Number

NP_006155.2

Uniprot Accession Number

Q16236

Species

Human

AA Sequence

MMDLELPPPG LPSQQDMDLI DILWRQDIDL GVSREVFDFS QRRKEYELEK QKKLEKERQE QLQKEQEKAF FAQLQLDEET GEFLPIQPAQ HIQSETSGSA NYSQVAHIPK SDALYFDDCM QLLAQTFPFV DDNEVSSATF QSLVPDIPGH IESPVFIATN QAQSPETSVA QVAPVDLDGM QQDIEQVWEE LLSIPELQCL NIENDKLVET TMVPSPEAKL TEVDNYHFYS SIPSMEKEVG NCSPHFLNAF EDSFSSILST EDPNQLTVNS LNSDATVNTD FGDEFYSAFI AEPSISNSMP SPATLSHSLS ELLNGPIDVS DLSLCKAFNQ NHPESTAEFN DSDSGISLNT SPSVASPEHS VESSSYGDTL LGLSDSEVEE LDSAPGSVKQ NGPKTPVHSS GDMVQPLSPS QGQSTHVHDA QCENTPEKEL PVSPGHRKTP FTKDKHSSRL EAHLTRDELR AKALHIPFPV EKIINLPVVD FNEMMSKEQF NEAQLALIRD IRRRGKNKVA AQNCRKRKLE NIVELEQDLD HLKDEKEKLL KEKGENDKSL HLLKKQLSTL YLEVFSMLRD EDGKPYSPSE YSLQQTRDGN VFLVPKSKKP DVKKN