Recombinant human THOC7 protein
THOC7 is required for efficient export of polyadenylated RNA. This protein acts as component of the THO subcomplex of the TREX complex which is thought to couple mRNA transcription, processing and nuclear export, and which specifically associates with spliced mRNA and not with unspliced pre-mRNA. TREX is recruited to spliced mRNAs by a transcription-independent mechanism, binds to mRNA upstream of the exon-junction complex (EJC) and is recruited in a splicing- and cap-dependent manner to a region near the 5' end of the mRNA where it functions in mRNA export to the cytoplasm via the TAP/NFX1 pathway. The TREX complex is essential for the export of Kaposi's sarcoma-associated herpesvirus (KSHV) intronless mRNAs and infectious virus production. Recombinant human THOC7 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
THO complex subunit 7 homolog, fSAP24, hTREX30, NIF3L1BP1
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
26.1 kDa (227aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
El Bounkari O., et al. (2009) FEBS Lett. 583 (1), 13-18
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) containing, 50% glycerol, 1mM DTT
Scientific Category
Epigenetics and Nuclear Signaling
NCBI Accession Number
NP_079351
Uniprot Accession Number
Q6I9Y2
Species
Human
AA Sequence
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