Recombinant human Wnt-3a protein

The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. WNT3a is a member of the WNT gene family. It encodes a protein which shows 96% amino acid identity to mouse Wnt3A protein, and 84% to human WNT3 protein, another WNT gene product. This protein is clustered with WNT14 gene, another family member, in chromosome 1q42 region. Recombinant human WNT3a protein was expressed in E. coli.

Product Specifications

Product Name Alternative

Wnt3a, Wingless-type MMTV integration site family, member 3A

Expression System

E.coli

Antigen Species

Human

Tag

Non-Tagged

Applications

SDS-PAGE, Denatured

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

37.5 kDa (335aa)

Additionnal Information

WNT3a, Protein Wnt-3a, Protein Wnt-3a, wnt-3a, ATGP2939-10 µg, ATGP2939-20 µg, ATGP2939-50 µg, ATGP2939-100 µg, ATGP2939-250 µg, ATGP2939-500 µg, ATGP2939-1 mg, ATGP2939-10, ATGP2939-20, ATGP2939-50, ATGP2939-100, ATGP2939-250, ATGP2939-500, ATGP2939-1

References & Citations

Baenziger C., et al. (2006) . Cell 125:509-522; ; Bourhis E., et al. (2010) . J. Biol. Chem. 285:9172-9179;

Other References

Kondo S, et al. Establishment of a novel culture method for maintaining intestinal stem cells derived from human induced pluripotent stem cells. (Biol Open. 2020) {https://pubmed.ncbi.nlm.nih.gov/31919043/}

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol

Scientific Category

Neuroscience

NCBI Accession Number

NP_149122.1

Uniprot Accession Number

P56704

Species

Human

AA Sequence

MSYPIWWSLA VGPQYSSLGS QPILCASIPG LVPKQLRFCR NYVEIMPSVA EGIKIGIQEC QHQFRGRRWN CTTVHDSLAI FGPVLDKATR ESAFVHAIAS AGVAFAVTRS CAEGTAAICG CSSRHQGSPG KGWKWGGCSE DIEFGGMVSR EFADARENRP DARSAMNRHN NEAGRQAIAS HMHLKCKCHG LSGSCEVKTC WWSQPDFRAI GDFLKDKYDS ASEMVVEKHR ESRGWVETLR PRYTYFKVPT ERDLVYYEAS PNFCEPNPET GSFGTRDRTC NVSSHGIDGC DLLCCGRGHN ARAERRREKC RCVFHWCCYV SCQECTRVYD VHTCK