Recombinant human PDHX protein

The pyruvate dehydrogenase (PDH) complex is located in the mitochondrial matrix and catalyzes the conversion of pyruvate to acetyl coenzyme A. The PDH complex thereby links glycolysis to Krebs cycle. The PDH complex contains three catalytic subunits, E1, E2, and E3, two regulatory subunits, E1 kinase and E1 phosphatase, and a non-catalytic subunit, E3 binding protein (E3BP) . This gene encodes the E3 binding protein subunit; also known as component X of the pyruvate dehydrogenase complex. This protein tethers E3 dimers to the E2 core of the PDH complex. Recombinant human PDHX protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Pyruvate dehydrogenase protein X component mitochondrial, Pyruvate dehydrogenase protein X component, mitochondrial, DLDBP, E3BP, OPDX, PDX1, proX

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

50.4 kDa (471aa)

Additionnal Information

PDHX, Pyruvate dehydrogenase protein X component mitochondrial, Pyruvate dehydrogenase protein X component, mitochondrial, DLDBP, E3BP, OPDX, PDX1, proX, ATGP2937-10 µg, ATGP2937-20 µg, ATGP2937-50 µg, ATGP2937-100 µg, ATGP2937-250 µg, ATGP2937-500 µg, ATGP2937-1 mg, ATGP2937-10, ATGP2937-20, ATGP2937-50, ATGP2937-100, ATGP2937-250, ATGP2937-500, ATGP2937-1

References & Citations

Aral B., et al. (1997) Am. J. Hum. Genet. 61:1318-1326; ; Hiromasa Y., et al. (2004) J. Biol. Chem. 279:6921-6933;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 20% glycerol, 1mM DTT

Scientific Category

Metabolism

NCBI Accession Number

NP_003468

Uniprot Accession Number

O00330

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGSGDPIKIL MPSLSPTMEE GNIVKWLKKE GEAVSAGDAL CEIETDKAVV TLDASDDGIL AKIVVEEGSK NIRLGSLIGL IVEEGEDWKH VEIPKDVGPP PPVSKPSEPR PSPEPQISIP VKKEHIPGTL RFRLSPAARN ILEKHSLDAS QGTATGPRGI FTKEDALKLV QLKQTGKITE SRPTPAPTAT PTAPSPLQAT AGPSYPRPVI PPVSTPGQPN AVGTFTEIPA SNIRRVIAKR LTESKSTVPH AYATADCDLG AVLKVRQDLV KDDIKVSVND FIIKAAAVTL KQMPDVNVSW DGEGPKQLPF IDISVAVATD KGLLTPIIKD AAAKGIQEIA DSVKALSKKA RDGKLLPEEY QGGSFSISNL GMFGIDEFTA VINPPQACIL AVGRFRPVLK LTEDEEGNAK LQQRQLITVT MSSDSRVVDD ELATRFLKSF KANLENPIRL A