Recombinant human ZFAND5 protein
ZFAND5 is involved in protein degradation via the ubiquitin-proteasome system. This protein may act by anchoring ubiquitinated proteins to the proteasome and plays a role in ubiquitin-mediated protein degradation during muscle atrophy. ZFAND5 plays a role in the regulation of NF-kappa-B activation and apoptosis and inhibits NF-kappa-B activation triggered by overexpression of RIPK1 and TRAF6 but not of RELA. It inhibits also tumor necrosis factor (TNF), IL-1 and TLR4-induced NF-kappa-B activation in a dose-dependent manner. Recombinant human ZFAND5 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
AN1-type zinc finger protein 5, AN1-type zinc finger protein 5, ZA20D2, ZFAND5A, ZNF216
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 85% by SDS-PAGE
Molecular Weight
25.5 kDa (236aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)
Additionnal Information
References & Citations
Huang J., et al. (2004) J. Biol. Chem. 279:16847-16853.; ; Gauci S., et al. (2009) Anal. Chem. 81:4493-4501.;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid In. Phosphate-Buffered Saline (pH 7.4) containing 30% glycerol, 1mM DTT
Scientific Category
Apoptosis
NCBI Accession Number
NP_005998
Uniprot Accession Number
O76080
Species
Human
AA Sequence
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