Recombinant human ZFAND5 protein

ZFAND5 is involved in protein degradation via the ubiquitin-proteasome system. This protein may act by anchoring ubiquitinated proteins to the proteasome and plays a role in ubiquitin-mediated protein degradation during muscle atrophy. ZFAND5 plays a role in the regulation of NF-kappa-B activation and apoptosis and inhibits NF-kappa-B activation triggered by overexpression of RIPK1 and TRAF6 but not of RELA. It inhibits also tumor necrosis factor (TNF), IL-1 and TLR4-induced NF-kappa-B activation in a dose-dependent manner. Recombinant human ZFAND5 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

AN1-type zinc finger protein 5, AN1-type zinc finger protein 5, ZA20D2, ZFAND5A, ZNF216

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

25.5 kDa (236aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

Additionnal Information

ZFAND5, AN1-type zinc finger protein 5, AN1-type zinc finger protein 5, ZA20D2, ZFAND5A, ZNF216, ATGP2931-10 µg, ATGP2931-20 µg, ATGP2931-50 µg, ATGP2931-100 µg, ATGP2931-250 µg, ATGP2931-500 µg, ATGP2931-1 mg, ATGP2931-10, ATGP2931-20, ATGP2931-50, ATGP2931-100, ATGP2931-250, ATGP2931-500, ATGP2931-1

References & Citations

Huang J., et al. (2004) J. Biol. Chem. 279:16847-16853.; ; Gauci S., et al. (2009) Anal. Chem. 81:4493-4501.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid In. Phosphate-Buffered Saline (pH 7.4) containing 30% glycerol, 1mM DTT

Scientific Category

Apoptosis

NCBI Accession Number

NP_005998

Uniprot Accession Number

O76080

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGSMAQETNQ TPGPMLCSTG CGFYGNPRTN GMCSVCYKEH LQRQQNSGRM SPMGTASGSN SPTSDSASVQ RADTSLNNCE GAAGSTSEKS RNVPVAALPV TQQMTEMSIS REDKITTPKT EVSEPVVTQP SPSVSQPSTS QSEEKAPELP KPKKNRCFMC RKKVGLTGFD CRCGNLFCGL HRYSDKHNCP YDYKAEAAAK IRKENPVVVA EKIQRI