Recombinant human PICK1 protein

PICK1 contains a PDZ domain, through which it interacts with protein kinase C, alpha (PRKCA) . This protein may function as an adaptor that binds to and organizes the subcellular localization of a variety of membrane proteins. It has been shown to interact with multiple glutamate receptor subtypes, monoamine plasma membrane transporters, as well as non-voltage gated sodium channels, and may target PRKCA to these membrane proteins and thus regulate their distribution and function. PICK1 has also been found to act as an anchoring protein that specifically targets PRKCA to mitochondria in a ligand-specific manner. Recombinant human PICK1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

PRKCA-binding protein, Protein interacting with PRKCA 1, PICK, PRKCABP

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

49 kDa (438aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

Additionnal Information

PICK1, PRKCA-binding protein, Protein interacting with PRKCA 1, PICK, PRKCABP, ATGP2927-10 µg, ATGP2927-20 µg, ATGP2927-50 µg, ATGP2927-100 µg, ATGP2927-250 µg, ATGP2927-500 µg, ATGP2927-1 mg, ATGP2927-10, ATGP2927-20, ATGP2927-50, ATGP2927-100, ATGP2927-250, ATGP2927-500, ATGP2927-1

References & Citations

Shao X., et al. (2010) Neurochem. Int. 56:962-970.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 30% glycerol, 1mM DTT

Scientific Category

Neuroscience

NCBI Accession Number

NP_001034673

Uniprot Accession Number

Q9NRD5

Species

Human

AA Sequence

MFADLDY DIEEDKLGIP TVPGKVTLQK DAQNLIGISI GGGAQYCPCL YIVQVFDNTP AALDGTVAAG DEITGVNGRS IKGKTKVEVA KMIQEVKGEV TIHYNKLQAD PKQGMSLDIV LKKVKHRLVE NMSSGTADAL GLSRAILCND GLVKRLEELE RTAELYKGMT EHTKNLLRAF YELSQTHRAF GDVFSVIGVR EPQPAASEAF VKFADAHRSI EKFGIRLLKT IKPMLTDLNT YLNKAIPDTR LTIKKYLDVK FEYLSYCLKV KEMDDEEYSC IALGEPLYRV STGNYEYRLI LRCRQEARAR FSQMRKDVLE KMELLDQKHV QDIVFQLQRL VSTMSKYYND CYAVLRDADV FPIEVDLAHT TLAYGLNQEE FTDGEEEEEE EDTAAGEPSR DTRGAAGPLD KGGSWCDS