Recombinant human B-Raf protein
BRAF belongs to the raf/mil family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this gene are associated with cardiofaciocutaneous syndrome, a disease characterized by heart defects, mental retardation and a distinctive facial appearance. Mutations in this gene have also been associated with various cancers, including non-Hodgkin lymphoma, colorectal cancer, malignant melanoma, thyroid carcinoma, non-small cell lung carcinoma, and adenocarcinoma of lung. A pseudogene, which is located on chromosome X, has been identified for this gene. Recombinant human BRAF protein, fused to His-tag at N-terminus, was expressed in E. coli.
Product Specifications
Product Name Alternative
Serine/threonine-protein kinase B-raf, Serine/threonine-protein kinase B-raf, B-RAF1, BRAF1, NS7, RAFB1
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE, Denatured
Concentration
0.25 mg/mL (determined by Bradford assay)
Purity
> 80% by SDS-PAGE
Molecular Weight
40.6 kDa (360aa)
Additionnal Information
References & Citations
Brennan D.F., et al. (2011) Nature 472:366-369
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol
Scientific Category
Apoptosis
NCBI Accession Number
NP_004324.2
Uniprot Accession Number
P15056
Species
Human
AA Sequence
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