Recombinant human Akt1 protein
AKT1 serine-threonine protein kinase is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Recombinant human AKT1 protein, fused to His-tag at N-terminus, was expressed in E. coli.
Product Specifications
Product Name Alternative
Protein kinase B, Protein kinase B alpha, Proto-oncogene c-Akt, RAC-PK-alpha
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE, Denatured
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 85% by SDS-PAGE
Molecular Weight
58.1 kDa (503aa)
Additionnal Information
References & Citations
Dobashi Y., et al. (2014) Hum. Pathol. 45 (1), 127-136; ; Liu K, et al. (2013) Mol. Cell. Biol. 33 (23), 4685-4700;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol
Scientific Category
Signal Transduction
NCBI Accession Number
NP_001014432
Uniprot Accession Number
P31749
Species
Human
AA Sequence
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