Recombinant human Opticin protein

Opticin belongs to class III of the small leucine-rich repeat protein (SLRP) family. Members of this family are typically associated with the extracellular matrix. Opticin is present in significant quantities in the vitreous of the eye and also localizes to the cornea, iris, ciliary body, optic nerve, choroid, retina, and fetal liver. Opticin may noncovalently bind collagen fibrils and regulate fibril morphology, spacing, and organization. The opticin gene is mapped to a region of chromosome 1 that is associated with the inherited eye diseases age-related macular degeneration (AMD) and posterior column ataxia with retinosa pigmentosa (AXPC1) . Recombinant human OPTC protein, fused to His-tag at N-terminus, was expressed in E. coli.

Product Specifications

Product Name Alternative

Opticin, Opticin, Opticin, OPT

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Denatured

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

37.6 kDa (336aa)

Additionnal Information

Opticin, Opticin, Opticin, Opticin, OPT, ATGP2914-10 µg, ATGP2914-20 µg, ATGP2914-50 µg, ATGP2914-100 µg, ATGP2914-250 µg, ATGP2914-500 µg, ATGP2914-1 mg, ATGP2914-10, ATGP2914-20, ATGP2914-50, ATGP2914-100, ATGP2914-250, ATGP2914-500, ATGP2914-1

References & Citations

Friedman J.S., et al. (2002) Hum. Mol. Genet. 11:1333-1342

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol

Scientific Category

Cytoskeleton

NCBI Accession Number

NP_055174

Uniprot Accession Number

Q9UBM4

Species

Human

AA Sequence

ASLPRKE RKRREEQMPR EGDSFEVLPL RNDVLNPDNY GEVIDLSNYE ELTDYGDQLP EVKVTSLAPA TSISPAKSTT APGTPSSNPT MTRPTTAGLL LSSQPNHGLP TCLVCVCLGS SVYCDDIDLE DIPPLPRRTA YLYARFNRIS RIRAEDFKGL TKLKRIDLSN NLISSIDNDA FRLLHALQDL ILPENQLEAL PVLPSGIEFL DVRLNRLQSS GIQPAAFRAM EKLQFLYLSD NLLDSIPGPL PLSLRSVHLQ NNLIETMQRD VFCDPEEHKH TRRQLEDIRL DGNPINLSLF PSAYFCLPRL PIGRFT