Recombinant human PLA2G7/PAF_AH/Lp_PLA2 protein

PLA2G7 (platelet-activating factor acetylhydrolase), also known as lipoprotein-associated phospholipase A2, is a phospholipase A2 enzyme. It is a secreted enzyme which catalyzes the degradation of platelet-activating factor to biologically inactive products. This protein is produced by inflammatory cells and hydrolyzes oxidised phospholipids in LDL. In the blood, PLA2G7 goes mainly with LDL and less than 20% is coupled with HDL. It is implicated in the development of atherosclerosis and is also a marker for cardiac disease. It might have a major physiologic effect in the presence of inflammatory bodily responses. Recombinant human PLA2G7 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.