Recombinant human Serpin B8/Proteinase Inhibitor 8 protein

The superfamily of high molecular weight serine proteinase inhibitors (serpins) regulate a diverse set of intracellular and extracellular processes such as complement activation, fibrinolysis, coagulation, cellular differentiation, tumor suppression, apoptosis, and cell migration. SERPINB8 is a member of the ov-serpin subfamily, which, relative to the archetypal serpin PI1, is characterized by a high degree of homology to chicken ovalbumin, lack of N- and C-terminal extensions, absence of a signal peptide, and a serine rather than an asparagine residue at the penultimate position. Recombinant human SERPINB8 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Serpin B8, Serpin B8, CAP2, PI8

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

45.2 kDa (397aa) confirmed by MALDI-TOF

Additionnal Information

Serpin B8, Proteinase Inhibitor8, Serpin B8, Serpin B8, CAP2, PI8, ATGP2909-10 µg, ATGP2909-20 µg, ATGP2909-50 µg, ATGP2909-100 µg, ATGP2909-250 µg, ATGP2909-500 µg, ATGP2909-1 mg, ATGP2909-10, ATGP2909-20, ATGP2909-50, ATGP2909-100, ATGP2909-250, ATGP2909-500, ATGP2909-1

References & Citations

Sprecher C.A., et al. (1995) J. Biol. Chem. 270:29854-29861.; ; Burkard T.R., et al. (2011) BMC Syst. Biol. 5:17-17.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 30% glycerol, 1mM DTT

Scientific Category

Cardiovascular

NCBI Accession Number

NP_942130

Uniprot Accession Number

P50452

Species

Human

AA Sequence

MDDLCEA NGTFAISLFK ILGEEDNSRN VFFSPMSISS ALAMVFMGAK GSTAAQMSQA LCLYKDGDIH RGFQSLLSEV NRTGTQYLLR TANRLFGEKT CDFLPDFKEY CQKFYQAELE ELSFAEDTEE CRKHINDWVA EKTEGKISEV LDAGTVDPLT KLVLVNAIYF KGKWNEQFDR KYTRGMLFKT NEEKKTVQMM FKEAKFKMGY ADEVHTQVLE LPYVEEELSM VILLPDDNTD LAVVEKALTY EKFKAWTNSE KLTKSKVQVF LPRLKLEESY DLEPFLRRLG MIDAFDEAKA DFSGMSTEKN VPLSKVAHKC FVEVNEEGTE AAAATAVVRN SRCSRMEPRF CADHPFLFFI RHHKTNCILF CGRFSSP