Recombinant human MRPL2 protein

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. MRPL2 is a 39S subunit protein that belongs to the EcoL2 ribosomal protein family. Recombinant human MRPL2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

39S ribosomal protein L2 mitochondrial, 39S ribosomal protein L2, mitochondrial, CGI-22, MRP-L14, RPML14

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

15.5 kDa (142aa) confirmed by MALDI-TOF

Additionnal Information

MRPL2, 39S ribosomal protein L2 mitochondrial, 39S ribosomal protein L2, mitochondrial, CGI-22, MRP-L14, RPML14, ATGP2904-10 µg, ATGP2904-20 µg, ATGP2904-50 µg, ATGP2904-100 µg, ATGP2904-250 µg, ATGP2904-500 µg, ATGP2904-1 mg, ATGP2904-10, ATGP2904-20, ATGP2904-50, ATGP2904-100, ATGP2904-250, ATGP2904-500, ATGP2904-1

References & Citations

O'Brien TW, Fiesler SE, et al. (1999) . J Biol Chem. 274 (51) :36043-51.; ; Kenmochi N, Suzuki T, et al. (2001) . Genomics. 77 (1-2) :65-70.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Phosphate buffer (pH 8.0) containing 1mM EDTA, 50% glycerol, 2mM DTT

Scientific Category

Epigenomics (Transcription & Translation)

NCBI Accession Number

NP_057034

Uniprot Accession Number

Q5T653

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGSGRDHTGR IRVHGIGGGH KQRYRMIDFL RFRPEETKSG PFEEKVIQVR YDPCRSADIA LVAGGSRKRW IIATENMQAG DTILNSNHIG RMAVAAREGD AHPLGALPVG TLINNVESEP GR