Recombinant human VCPKMT protein

VCPKMT is a protein-lysine N-methyltransferase that specifically trimethylates 'Lys-315' of VCP/p97; this modification may decrease VCP ATPase activity. Recombinant human VCPKMT protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Protein-lysine methyltransferase METTL21D, C14orf138, METTL21D, VCP-KMT

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

28.2 kDa (252aa) confirmed by MALDI-TOF

Additionnal Information

VCPKMT, Protein-lysine methyltransferase METTL21D, C14orf138, METTL21D, VCP-KMT, ATGP2903-10 µg, ATGP2903-20 µg, ATGP2903-50 µg, ATGP2903-100 µg, ATGP2903-250 µg, ATGP2903-500 µg, ATGP2903-1 mg, ATGP2903-10, ATGP2903-20, ATGP2903-50, ATGP2903-100, ATGP2903-250, ATGP2903-500, ATGP2903-1

References & Citations

Kernstock S., et al. (2012) Nat. Commun. 3:1038-1038.; ; Cloutier P., et al. (2013) PLoS Genet. 9:E1003210-E1003210.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid In. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Scientific Category

Other

NCBI Accession Number

NP_078834

Uniprot Accession Number

Q9H867

Species

Human

AA Sequence

MADTLES SLEDPLRSFV RVLEKRDGTV LRLQQYSSGG VGCVVWDAAI VLSKYLETPE FSGDGAHALS RRSVLELGSG TGAVGLMAAT LGADVVVTDL EELQDLLKMN INMNKHLVTG SVQAKVLKWG EEIEGFPSPP DFILMADCIY YEESLEPLLK TLKDISGFET CIICCYEQRT MGKNPEIEKK YFELLQLDFD FEKIPLEKHD EEYRSEDIHI IYIRKKKSKF PS