Recombinant human PAX8 protein

Paired box protein Pax-8 isoform PAX8E, also known as PAX8, is expressed in the developing and adult thyroid, the developing secretory system and at lower levels, in the adult kidney. PAX8complexes with TTF-1 and TTF-2 to induce thyroid follicular cell differentiation and thyroid hormone biosynthesis by regulating the expression of sodium iodide symporter (NIS), thyroid peroxidase (TPO), thyroglobulin (TG) and the thyrotropin receptor (TSHR) . Defects in PAX8 are the cause of congenital hypothyroidism non-goitrous type 2 (CHNG2) . Recombinant human PAX8 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Paired box protein Pax-8 isoform PAX8E, Paired box protein Pax-8 isoform PAX8E, paired box 8

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

33.4 kDa (310aa) confirmed by MALDI-TOF

Additionnal Information

PAX8, Paired box protein Pax-8 isoform PAX8E, Paired box protein Pax-8 isoform PAX8E, paired box 8, ATGP2898-10 µg, ATGP2898-20 µg, ATGP2898-50 µg, ATGP2898-100 µg, ATGP2898-250 µg, ATGP2898-500 µg, ATGP2898-1 mg, ATGP2898-10, ATGP2898-20, ATGP2898-50, ATGP2898-100, ATGP2898-250, ATGP2898-500, ATGP2898-1

References & Citations

Paters H., et al. (1998) Eur J Oral Sci. 106: 38-43.; ; Poleev A., et al. (1995) Eur J Biochem. 228: 899-911.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 20% glycerol , 1mM DTT

Scientific Category

Neuroscience

NCBI Accession Number

NP_054698.1

Uniprot Accession Number

Q06710

Species

Human

AA Sequence

MPHNSIR SGHGGLNQLG GAFVNGRPLP EVVRQRIVDL AHQGVRPCDI SRQLRVSHGC VSKILGRYYE TGSIRPGVIG GSKPKVATPK VVEKIGDYKR QNPTMFAWEI RDRLLAEGVC DNDTVPSVSS INRIIRTKVQ QPFNLPMDSC VATKSLSPGH TLIPSSAVTP PESPQSDSLG STYSINGLLG IAQPGSDKRK MDDSDQDSCR LSIDSQSSSS GPRKHLRTDA FSQHHLEPLE CPFERQHYPE AYASPSHTKG EQEVNTLAMP MATPPTPPTA RPGASPTPAC