Recombinant human RNA polymerases I, II, and III subunit RPABC4/POLR2K protein
POLR2K is one of the smallest subunits of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit is shared by the other two DNA-directed RNA polymerases. Recombinant human POLR2K protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 85% by SDS-PAGE
Molecular Weight
9.4 kDa (81aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Shpakovski G.V., et al. (1995) Mol. Cell. Biol. 15:4702-4710
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol, 1mM DTT
Scientific Category
Epigenomics (Transcription & Translation)
NCBI Accession Number
NP_005025
Uniprot Accession Number
P53803
Species
Human
AA Sequence
MDTQKDV QPPKQQPMIY ICGECHTENE IKSRDPIRCR ECGYRIMYKK RTKRLVVFDA R
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