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Recombinant human RNA polymerases I, II, and III subunit RPABC4/POLR2K protein

POLR2K is one of the smallest subunits of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit is shared by the other two DNA-directed RNA polymerases. Recombinant human POLR2K protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

DNA-directed RNA polymerases I II and III subunit RPABC4, DNA-directed RNA polymerases I, II, and III subunit RPABC4, ABC10-alpha, hRPB7.0, hsRPB10a, RPABC4, RPB10alpha, RPB12, RPB7.0

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

9.4 kDa (81aa) confirmed by MALDI-TOF

Additionnal Information

POLR2K, DNA-directed RNA polymerases I II and III subunit RPABC4, DNA-directed RNA polymerases I, II, and III subunit RPABC4, ABC10-alpha, hRPB7.0, hsRPB10a, RPABC4, RPB10alpha, RPB12, RPB7.0, ATGP2892-10 µg, ATGP2892-20 µg, ATGP2892-50 µg, ATGP2892-100 µg, ATGP2892-250 µg, ATGP2892-500 µg, ATGP2892-1 mg, ATGP2892-10, ATGP2892-20, ATGP2892-50, ATGP2892-100, ATGP2892-250, ATGP2892-500, ATGP2892-1

References & Citations

Shpakovski G.V., et al. (1995) Mol. Cell. Biol. 15:4702-4710

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol, 1mM DTT

Scientific Category

Epigenomics (Transcription & Translation)

NCBI Accession Number

NP_005025

Uniprot Accession Number

P53803

Species

Human

AA Sequence

MDTQKDV QPPKQQPMIY ICGECHTENE IKSRDPIRCR ECGYRIMYKK RTKRLVVFDA R

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