Recombinant human UBE2G1 protein
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. uE2G1 is a member of the E2 ubiquitin-conjugating enzyme family and catalyzes the covalent attachment of ubiquitin to other proteins. The protein may be involved in degradation of muscle-specific proteins. Recombinant human uBE2G1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Ubiquitin-conjugating enzyme E2 G1, E2 ubiquitin-conjugating enzyme G1, E217K, UBC7, Ubiquitin carrier protein G1, Ubiquitin-protein ligase G1, UBE2G
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 95% by SDS-PAGE
Molecular Weight
21.9 kDa (193aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Shibata, E., et al. (2011) Mol. Cell. Biol. 31 (15), 3136-3145; ; Hassink, G., et al. (2005) Biochem. J. 388 (PT 2), 647-655;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) containing, 30% glycerol, 1mM DTT
Scientific Category
Ubiquitination
NCBI Accession Number
NP_003333
Uniprot Accession Number
P62253
Species
Human
AA Sequence
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