Recombinant human ASRGL1 protein

Isoaspartyl peptidase/L-asparaginase, also known as ASRGL1, is a 308 amino acid protein belonging to the Ntn-hydrolase family. ASRGL1 has been identified as an autoantigenic protein that is present in the mid-piece of sperm after obstruction of the male reproductive tract. It is expressed highly in testis, but is also expressed in brain, kidney and gastrointestinal tissues. High levels of ASRGL1 have also been identified in ovarian, uterine and mammary tumors in comparison with normal tissues of the same origin. Recombinant human ASRGL1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Isoaspartyl peptidase/L-asparaginase, ALP, ALP1, CRASH

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

34.4 kDa (331aa) confirmed by MALDI-TOF

Additionnal Information

ASRGL1, Isoaspartyl peptidase/L-asparaginase, ALP, ALP1, CRASH, ATGP2889-10 µg, ATGP2889-20 µg, ATGP2889-50 µg, ATGP2889-100 µg, ATGP2889-250 µg, ATGP2889-500 µg, ATGP2889-1 mg, ATGP2889-10, ATGP2889-20, ATGP2889-50, ATGP2889-100, ATGP2889-250, ATGP2889-500, ATGP2889-1

References & Citations

Herr J C., et al. (1999) Biol Reprod. 61: 428-435.; ; Bush L A., et al. (2002) Mol Reprod Dev. 62: 233-247.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol , 1mM DTT

Scientific Category

Neuroscience

NCBI Accession Number

NP_001077395

Uniprot Accession Number

Q7L266

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGSMNPIVVV HGGGAGPISK DRKERVHQGM VRAATVGYGI LREGGSAVDA VEGAVVALED DPEFNAGCGS VLNTNGEVEM DASIMDGKDL SAGAVSAVQC IANPIKLARL VMEKTPHCFL TDQGAAQFAA AMGVPEIPGE KLVTERNKKR LEKEKHEKGA QKTDCQKNLG TVGAVALDCK GNVAYATSTG GIVNKMVGRV GDSPCLGAGG YADNDIGAVS TTGHGESILK VNLARLTLFH IEQGKTVEEA ADLSLGYMKS RVKGLGGLIV VSKTGDWVAK WTSTSMPWAA AKDGKLHFGI DPDDTTITDL P