Recombinant human NFU1 protein
NFU1 is a protein that is localized to mitochondria and plays a critical role in iron-sulfur cluster biogenesis. This protein assembles and transfers 4Fe-4S clusters to target apoproteins including succinate dehydrogenase and lipoic acid synthase. Mutations in this gene are a cause of bmultiple mitochondrial dysfunctions syndrome-1, and pseudogenes of this gene are located on the short arms of chromosomes 1 and 3. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. Recombinant human NFU1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
NFU1 iron-sulfur cluster scaffold, HIRA interacting protein 5, NFU1 iron-sulfur cluster scaffold homolog, HIRIP5, CGI-33, NifU, NIFUC
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
29.9 kDa (268aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Ganesh S., et al (2003) . Hum. Mol. Genet. 12:2359-2368; ; Tong W.-H., et al (2003) . Proc. Natl. Acad. Sci. u.S.A. 100:9762-9767;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 20% glycerol
Scientific Category
Other
NCBI Accession Number
NP_001002755.1
Uniprot Accession Number
Q9UMS0
Species
Human
AA Sequence
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