Recombinant human NT5C3B protein

NT5C3B, also known 7-methylguanosine phosphate-specific 5'-nucleotidase, includes transferase activity and nucleotide binding. Among its related super-pathways are adenosine nucleotides degradation II and Pyrimidine metabolism. Specifically it hydrolyzes 7-methylguanosine monophosphate (m (7) GMP) to 7-methylguanosine and inorganic phosphate. The specific activity for m (7) GMP may protect cells against undesired salvage of m (7) GMP and its incorporation into nucleic acids. Also it has weak activity for CMP. Recombinant human NT5C3B protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Product Specifications

Product Name Alternative

7-methylguanosine phosphate-specific 5'-nucleotidase, NT5C3L

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

36.8 kDa (323aa) confirmed by MALDI-TOF

Additionnal Information

NT5C3B, 7-methylguanosine phosphate-specific 5'-nucleotidase, NT5C3L, ATGP2887-10 µg, ATGP2887-20 µg, ATGP2887-50 µg, ATGP2887-100 µg, ATGP2887-250 µg, ATGP2887-500 µg, ATGP2887-1 mg, ATGP2887-10, ATGP2887-20, ATGP2887-50, ATGP2887-100, ATGP2887-250, ATGP2887-500, ATGP2887-1

References & Citations

Brieger A. et al. (2010) Proteomics. 10:3343-3355.; ; Aksoy P. et al. (2009) Pharmacogenet Genomics. 19:567-576.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 20% glycerol, 1mM DTT

Scientific Category

Epigenomics (Transcription & Translation)

NCBI Accession Number

NP_443167

Uniprot Accession Number

Q969T7

Species

Human

AA Sequence

MAEEVST LMKATVLMRQ PGRVQEIVGA LRKGGGDRLQ VISDFDMTLS RFAYNGKRCP SSYNILDNSK IISEECRKEL TALLHHYYPI EIDPHRTVKE KLPHMVEWWT KAHNLLCQQK IQKFQIAQVV RESNAMLREG YKTFFNTLYH NNIPLFIFSA GIGDILEEII RQMKVFHPNI HIVSNYMDFN EDGFLQGFKG QLIHTYNKNS SACENSGYFQ QLEGKTNVIL LGDSIGDLTM ADGVPGVQNI LKIGFLNDKV EERRERYMDS YDIVLEKDET LDVVNGLLQH ILCQGVQLEM QGP