Recombinant human NT5C3B protein
NT5C3B, also known 7-methylguanosine phosphate-specific 5'-nucleotidase, includes transferase activity and nucleotide binding. Among its related super-pathways are adenosine nucleotides degradation II and Pyrimidine metabolism. Specifically it hydrolyzes 7-methylguanosine monophosphate (m (7) GMP) to 7-methylguanosine and inorganic phosphate. The specific activity for m (7) GMP may protect cells against undesired salvage of m (7) GMP and its incorporation into nucleic acids. Also it has weak activity for CMP. Recombinant human NT5C3B protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.
Product Specifications
Product Name Alternative
7-methylguanosine phosphate-specific 5'-nucleotidase, NT5C3L
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 85% by SDS-PAGE
Molecular Weight
36.8 kDa (323aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Brieger A. et al. (2010) Proteomics. 10:3343-3355.; ; Aksoy P. et al. (2009) Pharmacogenet Genomics. 19:567-576.;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 20% glycerol, 1mM DTT
Scientific Category
Epigenomics (Transcription & Translation)
NCBI Accession Number
NP_443167
Uniprot Accession Number
Q969T7
Species
Human
AA Sequence
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