Recombinant human SDHAF1 protein
Succinate dehydrogenase assembly factor 1, also known as SDHAF1, plays an essential role in succinate dehydrogenase complex (SDH) assembly, a complex involved in complex II of the mitochondrial electron transport chain. Probably this protein acts by participating in mitochondrial biosynthesis of iron-sulfur centers for complex II. Recombinant human SDHAF1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Succinate dehydrogenase assembly factor 1, LYRM8
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.25 mg/mL (determined by Bradford assay)
Purity
> 85% by SDS-PAGE
Molecular Weight
15.2 kDa (138aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Ghezzi D., et al. (2009) Nat. Genet. 41:654-656; ; Grimwood J., et al. (2004) Nature. 428:529-535;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) 30% glycerol, 2mM DTT, 0.1mM PMSF
Scientific Category
Other
NCBI Accession Number
NP_001036096
Uniprot Accession Number
A6NFY7
Species
Human
AA Sequence
MSRHSRL QRQVLSLYRD LLRAGRGKPG AEARVRAEFR QHAGLPRSDV LRIEYLYRRG RRQLQLLRSG HATAMGAFVR PRAPTGEPGG VGSQPDDGDS PRNPHDSTGA PETRPDGR
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