Recombinant human CDK2 protein
Cyclin-dependent kinase 2, also known as CDK2, phosphorylates CTNNB1, uSP37, p53/TP53, NPM1, CDK7, RB1, BRCA2, MYC, NPAT, EZH2. This protein acts at the G1-S transition to promote the E2F transcriptional program and the initiation of DNA synthesis, and modulates G2 progression; controls the timing of entry into mitosis/meiosis by controlling the subsequent activation of cyclin B/CDK1 by phosphorylation, and coordinates the activation of cyclin B/CDK1 at the centrosome and in the nucleus. This protein is crucial role in orchestrating a fine balance between cellular proliferation, cell death, and DNA repair in human embryonic stem cells (hESCs) . Recombinant human CDK2 protein, fused to His-tag at C-terminus, was expressed in insect cells using baculovirus expression system and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Cyclin-dependent kinase 2, p33 (CDK2)
Expression System
Baculovirus
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
34.9 kDa (306aa)
Additionnal Information
References & Citations
Okuda M., et al. (2000) Cell. 103:127-140.; ; Ma T., et al. (2000) Genes Dev. 14:2298-2313.;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) 30% glycerol, 2mM DTT, 0.1mM PMSF
Scientific Category
Cell Cycle
NCBI Accession Number
NP_001789.2
Uniprot Accession Number
P24941
Species
Human
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