Recombinant human CDK1 protein
Cyclin-dependent kinase 1, also known as CDK1, plays a key role in the control of the eukaryotic cell cycle by modulating the centrosome cycle as well as mitotic onset; promotes G2-M transition, and regulates G1 progress and G1-S transition via association with multiple interphase cyclins. This protein is required in higher cells for entry into S-phase and mitosis. Recombinant human CDK1 protein, fused to His-tag at C-terminus, was expressed in insect cells using baculovirus expression system and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Cyclin-dependent kinase 1, CDC2, CDC28A, P34CDC2
Expression System
Baculovirus
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 95% by SDS-PAGE
Molecular Weight
35.1 kDa (305aa)
Additionnal Information
References & Citations
Fourest-Lieuvin A., et al. (2006) Mol. Biol. Cell. 17:1041-1050; ; Westbrook L., et al. (2007) Cancer Res. 67:11393-11401;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) 30% glycerol, 2mM DTT, 0.1mM PMSF
Scientific Category
Cell Cycle
NCBI Accession Number
NP_001777
Uniprot Accession Number
P06493
Species
Human
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