Recombinant human VEGF 165 protein
Vascular endothelial growth factor A, also known as VEGF, is growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. This protein binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. NRP1/Neuropilin-1 binds isoforms VEGF-165 and VEGF-145. Isoform VEGF165B binds to KDR but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth. Recombinant human VEGF165 protein, fused to His-tag at C-terminus, was expressed in insect cells using baculovirus expression system and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Vascular endothelial growth factor A, Vascular endothelial growth factor A, MVCD1, VEGF, VPF
Expression System
Baculovirus
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.25 mg/mL (determined by Bradford assay)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 85% by SDS-PAGE
Molecular Weight
19.9 kDa (171aa)
Additionnal Information
References & Citations
Woolard J., et al. (2004) Cancer Res. 64:7822-7835.; ; Dixelius J., et al. (2006) Cancer Res. 66:2089-2097.;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) 30% glycerol, 1mM DTT, 0.1mM PMSF
Scientific Category
Hormones & Cytokines
NCBI Accession Number
NP_001165097
Uniprot Accession Number
P15692
Species
Human
Explore Other Products
Discover premium biology products from our extensive collection of 20M+ items
