Recombinant human QTRTD1 protein
QTRTD1 interacts with QTRT1 to form an active queuine tRNA-ribosyltransferase. This enzyme exchanges queuine for the guanine at the wobble position of tRNAs with GuN anticodons (tRNA-Asp, -Asn, -His and -Tyr), thereby forming the hypermodified nucleoside queuosine. Recombinant human QTRTD1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.25 mg/mL (determined by Bradford assay)
Purity
> 95% by SDS-PAGE
Molecular Weight
49.1 kDa (438aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Chen, Y.C., et al. (2010) RNA 16 (5), 958-968
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 30% glycerol, 2mM DTT, 1mM EDTA, 0.1mM PMSF
Scientific Category
Epigenomics (Transcription & Translation)
NCBI Accession Number
NP_078914
Uniprot Accession Number
Q9H974
Species
Human
AA Sequence
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