Recombinant human QTRTD1 protein

QTRTD1 interacts with QTRT1 to form an active queuine tRNA-ribosyltransferase. This enzyme exchanges queuine for the guanine at the wobble position of tRNAs with GuN anticodons (tRNA-Asp, -Asn, -His and -Tyr), thereby forming the hypermodified nucleoside queuosine. Recombinant human QTRTD1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Queuine tRNA-ribosyltransferase subunit QTRTD1, Queuine tRNA-ribosyltransferase subunit QTRTD1, FLJ12960, Queuine tRNA-ribosyltransferase domain-containing protein 1, Queuine tRNA ribosyltransferase subunit QTRTD1

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

49.1 kDa (438aa) confirmed by MALDI-TOF

Additionnal Information

QTRTD1, Queuine tRNA-ribosyltransferase subunit QTRTD1, Queuine tRNA-ribosyltransferase subunit QTRTD1, FLJ12960, Queuine tRNA-ribosyltransferase domain-containing protein 1, Queuine tRNA ribosyltransferase subunit QTRTD1, ATGP2868-10 µg, ATGP2868-20 µg, ATGP2868-50 µg, ATGP2868-100 µg, ATGP2868-250 µg, ATGP2868-500 µg, ATGP2868-1 mg, ATGP2868-10, ATGP2868-20, ATGP2868-50, ATGP2868-100, ATGP2868-250, ATGP2868-500, ATGP2868-1

References & Citations

Chen, Y.C., et al. (2010) RNA 16 (5), 958-968

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 30% glycerol, 2mM DTT, 1mM EDTA, 0.1mM PMSF

Scientific Category

Epigenomics (Transcription & Translation)

NCBI Accession Number

NP_078914

Uniprot Accession Number

Q9H974

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGSMKLSLTK VVNGCRLGKI KNLGKTGDHT MDIPGCLLYT KTGSAPHLTH HTLHNIHGVP AMAQLTLSSL AEHHEVLTEY KEGVGKFIGM PESLLYCSLH DPVSPCPAGY VTNKSVSVWS VAGRVEMTVS KFMAIQKALQ PDWFQCLSDG EVSCKEATSI KRVRKSVDRS LLFLDNCLRL QEESEVLQKS VIIGVIEGGD VMEERLRSAR ETAKRPVGGF LLDGFQGNPT TLEARLRLLS SVTAELPEDK PRLISGVSRP DEVLECIERG VDLFESFFPY QVTERGCALT FSFDYQPNPE ETLLQQNGTQ EEIKCMDQIK KIETTGCNQE ITSFEINLKE KKYQEDFNPL VRGCSCYCCK NHTRAYIHHL LVTNELLAGV LLMMHNFEHY FGFFHYIREA LKSDKLAQLK ELIHRQAS