Recombinant human UGP2 protein

UGP2 is an important intermediary in mammalian carbohydrate interconversions. It transfers a glucose moiety from glucose-1-phosphate to MguTP and forms uDP-glucose and MgPPi. In liver and muscle tissue, uDP-glucose is a direct precursor of glycogen; in lactating mammary gland it is converted to uDP-galactose which is then converted to lactose. Recombinant human uGP2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

UDP-glucose pyrophosphorylase 2, UDP-glucose pyrophosphorylase 1, UTP--glucose-1-phosphate uridylyltransferase, UGP1, UGPP1, SVUGP2, UDPGP, UGPase

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

59.3 kDa (531aa)

Additionnal Information

SVUGP 2, SVUGP2, UDP glucose pyrophosphorylase, UDP glucose pyrophosphorylase 1, UDP glucose pyrophosphorylase 2, UDPG, UDP-glucose pyrophosphorylase, UDP-glucose pyrophosphorylase 1, UDP-glucose pyrophosphorylase 2, UDPGP, UDPGP 2, UDPGP2, UGP 1, UGP 2, UGP1, UGP2, UGPase, UGPP 1, UGPP1, UTP glucose 1 phosphate uridylyltransferase, UTP-glucose-1-phosphate uridylyltransferase, UTP--glucose-1-phosphate uridylyltransferase, ATGP2865-10 µg, ATGP2865-20 µg, ATGP2865-50 µg, ATGP2865-100 µg, ATGP2865-250 µg, ATGP2865-500 µg, ATGP2865-1 mg, ATGP2865-010, ATGP2865-020, ATGP2865-050, ATGP2865-100, ATGP2865-250, ATGP2865-500, ATGP2865-01M

References & Citations

Fuhring, J., et al. (2013) Glycobiology 23 (4), 426-437

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 30% glycerol,1mM DTT

Scientific Category

Metabolism

NCBI Accession Number

NP_006750.3

Uniprot Accession Number

Q16851

Species

Human

AA Sequence

MSRFVQD LSKAMSQDGA SQFQEVIRQE LELSVKKELE KILTTASSHE FEHTKKDLDG FRKLFHRFLQ EKGPSVDWGK IQRPPEDSIQ PYEKIKARGL PDNISSVLNK LVVVKLNGGL GTSMGCKGPK SLIGVRNENT FLDLTVQQIE HLNKTYNTDV PLVLMNSFNT DEDTKKILQK YNHCRVKIYT FNQSRYPRIN KESLLPVAKD VSYSGENTEA WYPPGHGDIY ASFYNSGLLD TFIGEGKEYI FVSNIDNLGA TVDLYILNHL MNPPNGKRCE FVMEVTNKTR ADVKGGTLTQ YEGKLRLVEI AQVPKAHVDE FKSVSKFKIF NTNNLWISLA AVKRLQEQNA IDMEIIVNAK TLDGGLNVIQ LETAVGAAIK SFENSLGINV PRSRFLPVKT TSDLLLVMSN LYSLNAGSLT MSEKREFPTV PLVKLGSSFT KVQDYLRRFE SIPDMLELDH LTVSGDVTFG KNVSLKGTVI IIANHGDRID IPPGAVLENK IVSGNLRILD H