Recombinant human MRPS23 protein

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. MRPS23 encodes a 28S subunit protein. Recombinant human MRPS23 protein, fused to His-tag at N-terminus, was expressed in E. coli.

Product Specifications

Product Name Alternative

28S ribosomal protein S23 mitochondrial, 28S ribosomal protein S23, mitochondrial, CGI-138, HSPC329, MRP-S23

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Denatured

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

24.2 kDa (213aa)

Additionnal Information

MRPS23, 28S ribosomal protein S23 mitochondrial, 28S ribosomal protein S23, mitochondrial, CGI-138, HSPC329, MRP-S23, ATGP2861-10 µg, ATGP2861-20 µg, ATGP2861-50 µg, ATGP2861-100 µg, ATGP2861-250 µg, ATGP2861-500 µg, ATGP2861-1 mg, ATGP2861-10, ATGP2861-20, ATGP2861-50, ATGP2861-100, ATGP2861-250, ATGP2861-500, ATGP2861-1

References & Citations

Lai C.-H., et al (2000) . Genome Res. 10:703-713; ; Kenmochi N., et al (2001) . Genomics 77:65-70;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Scientific Category

Epigenomics (Transcription & Translation)

NCBI Accession Number

NP_057154

Uniprot Accession Number

Q9Y3D9

Species

Human

AA Sequence

MAGSRLE TVGSIFSRTR DLVRAGVLKE KPLWFDVYDA FPPLREPVFQ RPRVRYGKAK APIQDIWYHE DRIRAKFYSV YGSGQRAFDL FNPNFKSTCQ RFVEKYTELQ KLGETDEEKL FVETGKALLA EGVILRRVGE ARTQHGGSHV SRKSEHLSVR PQTALEENET QKEVPQDQHL EAPADQSKGL LPP