Recombinant mouse IL-33 protein

Interleukin 33 (IL-33) is a 32kDa proinflammatory cytokine that may also regulate gene transcription in producer cells. IL-33 is structurally related to IL-1, which induces helper T cells to produce type 2 cytokines and acts through the receptor IL1RL-1 (IL1 receptor-like-1), which is known also as ST2. Binding of IL-33 to this receptor activates NF-kappa-B and MAP kinases and induces in vitro Th2 cells to produce cytokines. In vivo, IL-33 induces expression of IL-4, IL-5, IL-13 and leads to severe pathological changes in mucosal organs and in vitro, it can be divided to N-terminal fragment of 12kDa and C-terminal fragment of 18kDa by cleavage of caspase-1. Recombinant mouse IL33 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Specifications

Mouse IL-33 stimulates cell proliferation D10.G4.1 mouse helper T cells. The ED50 range ≤ 0.1 ng/mL.

Product Name Alternative

Interleukin-33, IL-33, Il1f11, NF-HEV, 230117N10Rik

Expression System

E.coli

Antigen Species

Mouse

Tag

His-Tag

Applications

SDS-PAGE, Bioactivity

Concentration

0.5 mg/mL (determined by Bradford assay)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 95% by SDS-PAGE

Bioactivity

Measured in a cell proliferation assay using D10.G4.1 mouse helper T cells. The ED50 range ≤ 0.1 ng/mL.

Molecular Weight

18.1 kDa (181aa) confirmed by MALDI-TOF

Additionnal Information

Interleukin-33, 230117N10Rik, IL-33, Il-33, Il1f11, Interleukin 33, NF-HEV, ATGP2860-10 µg, ATGP2860-20 µg, ATGP2860-50 µg, ATGP2860-100 µg, ATGP2860-250 µg, ATGP2860-500 µg, ATGP2860-1 mg, ATGP2860-010, ATGP2860-020, ATGP2860-050, ATGP2860-100, ATGP2860-250, ATGP2860-500, ATGP2860-01M

References & Citations

Schmitz J., et al (2005) Immunity 23:479-490; ; Ali S., et al (2011) J. Immunol. 187:1609-1616

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol, 1mM DTT

Scientific Category

Hormones & Cytokines

NCBI Accession Number

NP_001158196.1

Uniprot Accession Number

Q8BVZ5

Species

Mouse

AA Sequence

SIQGTSL LTQSPASLST YNDQSVSFVL ENGCYVINVD DSGKDQEQDQ VLLRYYESPC PASQSGDGVD GKKLMVNMSP IKDTDIWLHA NDKDYSVELQ RGDVSPPEQA FFVLHKKSSD FVSFECKNLP GTYIGVKDNQ LALVEEKDES CNNIMFKLSK I